Diagnosis of childhood UTIs is challenging in the outpatient setting. GP’s perspectives for the diagnostic workup of childhood UTIs are not well understood. In this study, we found that assuming low UTI prevalence, the aspecific presentation of UTI in children and difficulties in urine collection were barriers for diagnosis of childhood UTI. Diagnostic uncertainty makes appropriate treatment challenging. Factors that might improve the diagnostic workup were: novel noninvasive collection techniques, instructional material for the parents, skill training for GPs, decision support tools, accurate and easy-to-use point-of-care tests and guidance on urine culture interpretation.