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Episode Summary:

In this episode, we explore 5 Amino 1MQ, an investigational compound gaining attention for its potential effects on metabolism, body composition and healthy aging. We break down how it inhibits the NNMT enzyme, supports NAD+ availability and may influence lipolysis, fat oxidation and cellular energy production. We also discuss the current research, potential benefits, commonly discussed protocols, safety considerations and why human evidence remains limited.

Welcome back everyone.

I'm a little under the weather so I am hoping to make it through this recording because I know I have not put any content out in the last couple of weeks.

I have just been super busy.

I just got back from cousin camp about a week ago and I think that's where I got a little bug or something because there were 19 of us in one house and two of my cousin's children had babies, one had a set of twins and then all three of them are girls.

And so that was so fun to have the little babies around and new additions to our ever growing family.

My son officially just got engaged a couple of weeks ago.

We had a big engagement party here at the house and we could not be more excited for Logan and my future daughter in law, Caroline.

I definitely hit the jackpot with her, as did my son.

Super big congratulations to the two of them, although I know neither one of them listened to my podcast.

All that being said, there is some exciting stuff coming Coming up at the Peptique.

We are expanding our topical line and I'm hoping within the next month.

We just got the samples in.

I'm absolutely obsessed with it.

We are going to release a very potent GHKCU B body lotion.

I've been using it.

I absolutely love it.

Our face serum is one of our biggest sellers and we're going to transition into a whole topical line that is will be available at the Peptique but we're going to take it to a more traditional type of website where we can do processing normally all the things that I can't do with the Peptique into a whole new topical company.

Just stay tuned for that.

I'm jumping the gun a little bit by mentioning it here, but I'm just so excited.

And all that being said, you can imagine how busy that's kept me, which is why my recording has not been as frequent.

And I am about to take a extended, well needed trip with my husband out of the country for two weeks.

And so I'm going to try to get one more episode out next week and then I'll go silent again for a couple of weeks.

So just bear with me as I have all these things that I'm juggling.

But know if you need to reach me or you have questions or anything, you can always email me.

I get emails from all the time and I love answering your questions.

And so anyway, that's enough with the housekeeping and what's going on in my world.

Oh, football season started.

You.

You guys know I'm a huge college football person.

And although I'm not a UT fan, I cannot believe that Texas came back and beat Ohio State last weekend.

That was totally insane.

Baylor's off to a good start.

We almost beat Auburn.

We definitely beat our.

I think it was Prairie View, so it was an easier game, but we beat them last week.

So my, one of my really good friend's son is a freshman who's a quarterback for Baylor and so it's fun just with the idea that they might put him in so all the way around football season.

So excited for that.

Okay, I digress.

Let's get into today's episode and I chose this particular topic because it's one that I happen to be cycling right now.

I've cycled it numerous times and I think in this space, as you kind of go along, I think one of the mistakes that a lot of people make is that they visit a peptide, they try it.

I didn't get anything out of it.

And I think what we have to remember in this particular, it's not a peptide, but we'll go into that in a minute.

This, this particular one, five amino one mq, let's just throw it out there.

I'm going to refer to it as 5amino because 5amino 1 MQ is a mouthful.

But I think this particular one, people have mixed results with it.

But the, the protocols on it are all over the map and it's super confusing.

And so it's one that I get a lot of feedback on.

I didn't get anything out of it at all or oh my gosh, I loved it.

And so we're going to talk a little bit about that.

We're going to talk about my own experience with it, what anecdotally I see in people and what they report and what to stack it with and the whole nine yards.

So let's just kind of dive in.

Okay?

There's some big words that I'm going to try to get out here without like totally messing them up, so don't make fun of me.

But 5amino 1 was discovered through research into NMNT.

And that's an enzyme found at particularly high levels in fats cells.

And researchers observed that N activity was elevated in obesity and metabolic dysfunction.

So reducing nn, sorry, mt genetically in mice increased energy expenditure and mice with lower NMT activity were more resistant to diet induced obesity.

And NNMT uses nicotinamide and methyl donors that are connected to NAD and cellular energy metabolism.

So that led scientists to search for some sort of compound capable of blocking NMNT and researchers screened a series of small molecules derived from quinolinium and isoquinolinium structures and studied how changes to those structures affected nmnt inhibition.

And 5amino 1 MQ emerged as one of the strongest candidates.

Because it inhibited NMNT effectively, it entered fat cells successfully.

It reduced production of one methyl nicot nicotinamide, sorry, confirming NMNT inhibition and was relatively selective for NMNT over several related enzymes and increase NAD in SAM levels and culture and cultured fat cells.

Okay, so this is why this becomes an interesting compound to talk about because it's not actually a peptide.

So I want to clarify that because I definitely think there some confusion out there about that because you talk about being in the peptide space, but lots of times like nad, which is an enzyme and things like that, it's not.

We're not actually talking about peptides all the time in this space.

Sometimes we're talking about small molecules and enzymes and things like that.

And 5Amino definitely falls in that category.

But let's just dive right into what the research says because we don't have really any human data to go off of.

So we're going to talk about the data that we do have, which is largely or exclusively really mice and lab rats and mice studies.

And the researchers used mice that had developed obesity through a high fat diet.

And the mice were given 5amino 1 MQ at 20 milligrams per kilogram three times daily.

That's a lot.

And by, and this is by subcutaneous administration for 11 days.

So it was a short study.

This equals a total experimental exposure of 60 milligrams per kilogram per day.

This was a high dose animal research protocol and should not be converted directly into human dose.

So let me just say that we're going to get into that a little bit later.

At the end of the 11 day experiment, the researchers reported approximately 5.1% loss of starting body weight in the treated obese mice, approximately 35% reduction in white adipose tissue mass and more than 30% reduction in average fat cell cross sectional area.

It also showed more than 40% reduction in estimated fat cell volume.

It also showed a significant reduction in fasting total plasma cholesterol.

So there was no significant difference in average or cumulative food intake compared with controls.

So they really weren't messing with the diet at all.

The food intake result is one of the most interesting findings, because the treated mice did not appear to lose weight simply because they were eating less.

The researchers interpreted the findings as evidence that NMT inhibition altered adipose tissue metabolism and energy expenditure.

And in the cell culture portion of this research, fully differentiated fat cells express 37 times more NMT protein than undifferentiated pre fat cells.

So the mature fat cells also contained approximately 7.5 times more of one methyl nicotinamide, indicating much greater NNMT activity.

And when the mature fat cells were treated with 5amino 1 Mq, intracellular NAD increased approximately 1.2 to 1.6 times, depending on concentration.

SAM, an important methyl donor, also increased at selected concentrations and one methyl nicotinamide production decreased.

And these are all markers associated with fat synthesis and they were all reduced.

And this is the foundational study behind Most claims that 5amino 1 may influence fat mass independently of of appetite.

Okay, so this was a small number of mice treated for only 11 days.

They were administered three times daily by injection.

So just kind of keep that in mind as we're going through some of these studies.

The researchers used approximately 24 month old mice.

And this is an age that's far advanced for mice.

Near the end of their lifespan, their muscles were deliberately injured so the researchers could evaluate the ability of older muscle stem cells to activate, proliferate and repair damaged tissue.

Aging muscle expressed more and demonstrated impaired NAD metabolism and increased cellular senescence and reduced regenerative capacity.

So they induced this condition for these mice.

Okay, treatment with the 5Amino1MQ showed increased activation and proliferation of aged muscle stem cells, which is what we all want.

Improved differentiation and fusion of muscle cells, formation of larger regenerating muscle fibers, improved contractile function after injury, and reduced markers of muscle stem cell senescence.

It also showed improved NAD plus and Sirtuin 1 related cellular signaling.

So the regenerated muscle fibers and treated aged mice were reported to have nearly twice the cross sectional area observed in untreated age controls.

Peak muscle torque also increased by approximately 70% in their treated groups.

Okay, so again, we're talking about mice here, but those findings are pretty respectable if you really break it down and start thinking about it.

This was published in the Journal of Pharmaceutical and Biomedical Analysis, and it examined the pharmacokinetics.

The researchers evaluated intravenous and oral administration in rats and they reported estimated oral viability of approximately 38.4% and an oral elimination half life of approximately 6.9 hours.

The intravenous half life was approximately 3.8 hours and showed rapid absorption following administration.

So this study is important because it demonstrates that 5Amino 1 reached systemic circulation after oral administration in rats.

However, we can't directly correlate to this in half life in humans, but it is promising that it shows that Orly might have some bioavailability where a lot of the small molecule and peptide capsules that we talk about don't have that.

So again, just something to keep in mind, there is also a reduced calorie diet Study researchers examined whether NNMT inhibition could enhance the effects of switching obese mice from a high fat western style diet hello America to a lower calorie diet.

The mice received 5amino 1 Mq at 32mg per kg once daily by subcutaneous administration while undergoing the dietary intervention.

The combination of the diet change and NMT inhibition produces substantially greater reduction in fat mass than the diet change alone.

Reported changes from the baseline were approximately 29.3% reduction in fat mass this is with the diet in place, okay, and approximately 2.9% reduction in fat mass with just the diet change alone.

The researchers also identified changes in adipose tissue metabolites associated with lipid synthesis and energy metabolism.

In later analysis of this model found changes in the gut microbiome profile that was positive.

So we can't automatically extrapolate that if you have microbiome issues this is going to make your microbiome healthier.

But it is a little side note in this particular study that they did mention.

This study used 22 month old so these again older female mice divided in four groups sedentary plus saline sedentary plus 5amino 1 mq progressive weighted will exercise plus the saline basically the placebo and progressive weighted will exercise plus 5amino 1 mq.

The treated mice received 5amino at 10 milligrams per kilogram once daily by subcutaneous administration for eight weeks and the sedentary treated mice had approximately 40% greater grip strength than untreated sedentary control.

So the ones that were administered the 5amino one but no exercise had a 40% greater grip strength and then the treated mice that did have exercise had approximately 60% greater grip grip strength than untreated se sedentary controls.

So combining this with exercise produced additive improvements in certain functional measurements.

So that's important to think about because when you have diet and exercise, or I like to say nutrition over diet, where it needs to be, and really tuned in these peptides and these small molecules and these enzymes are only going to work better for you.

So I thought that was really an interesting takeaway here because we all want to see that easy button, but if we don't have those underlying things and check, it's only going to get you so far.

And these mice studies truly reflected that.

So I found that pretty interesting.

Something else I want to note to you is you can see the dosing as high 60mg per kg a day, as low as 10mg per kg a day, and some of them are multiple times a day, et cetera.

I think this also explains a lot of the confusion surrounding the dosing for 5Amino 1 MQ, which we're going to go into a little bit later.

But just kind of keep that in the back of your mind because the higher doses didn't necessarily yield better results.

So I think that's something to remember when we go into the dosing portion of this episode a little bit later.

Okay, so some of the legitimate takeaways from all of this.

The published research suggests that 5amino 1 Mq inhibits NNMT inside cultured fat cells.

It increased NAD in SAM in experimental fat cell models.

It reduced fat mass and body weight in diet induced obese mice without reducing food intake.

It improved muscle regeneration after injury in aged mice.

It enhanced selected measures of strength and muscle function in aged mice.

And oral absorption was demonstrated in rats.

Okay, so unfortunately we don't have any real human data on this, which in this space is pretty common.

And so you kind of wonder though, why not?

Why hasn't big pharma jumped on this?

If you have these really robust animal studies that seem really promising and, and that's just the beginning, right?

These are preclinical animal studies, so they only are going to go so far.

But I think that everyone is so focused on the, the GLP craze and all the thinks and all the amazing things that the research is showing.

Lots of human research is showing that these GLPs can do that.

Things like 5Amino 1 and Q kind of get forgotten or lost because they're not as, they're not as robust as the GLPs.

That doesn't mean they're not meaningful and they can't be useful tools for wellness and longevity.

But let's talk a little bit about that because I often get the question, can I replace my glp, take a, a break from my GLP and use five amino one?

And of course you can do that, but is it going to get you the same results and that sort of thing?

And the answer is no.

I find that it works well in conjunction with the glp.

So let's kind of break down the difference between the 2 because both 5Amino1 and GLP are discussed in the context of weight management and metabolic health, but they do very different things.

Okay, so the GLPs reduce appetite and food cravings, increase satiety after eating, slow gastric emptying, increase glucose dependent insulin secretion and reduce inappropriate glucagon secretion and improved blood glucose regulation.

Where 5amino increases NAD and SAM availability, it affects cellular energy expenditure, it reduces fat synthesis and storage, it reduces fat cell size, it can influence fat oxidation and metabolic flexibility.

It can even support muscle stem cell activity.

So 5amino 1 has potential to reduce body weight and fat mass without significantly changing food intake.

And that suggests a mechanism related more to energy metabolism rather than appetite suppression and food intake.

So there's a difference there.

Okay.

And that's why the GLP is going to impact things very differently than the 5amino one.

And they're not a replacement for, for each other, but they can be used stats together and work beautifully.

And we're going to go into stacks here in just a little bit, but because we don't have really any real human data, let's talk anecdotally what you can expect from 5Amino 1 because we just don't have those human studies.

So we have to go off of what are all the communities out there that we live in this health optimization and peptide space.

Okay.

Some people describe a gradual reduction in body fat or waist measurements, improved body composition without major appetite suppression, greater energy, particularly during exercise, improved workout endurance, better recovery between workouts.

Some people note a warmer body temperature or increased sweating, greater metabolic flexibility, easier fat loss when nutrition and resistance training are already consistent.

Improved fasting glucose or insulin markers of reduction in weight loss plateaus.

So for those dreaded stalls that we often run into and better preservation of muscle while dieting, which is important, particularly when you're on a glp.

Okay.

Some of the side effects, obviously an injection side, if you're doing the SEP Q method, ejection site irritation, headache, nausea, overstimulation, sleep disruption, increased sweating are no noticeable effect at all.

I'VE taken multiple rounds of 5amino 1 mq.

I've never personally experienced the negative side effects from it, but I always say this, this is unique to the individual who's taking it.

So we don't always respond the same way.

So just note that there can be some side effects to it.

It's just not something that I've personally experienced.

Okay, let's go into what to stack it with.

MOT C is a no brainer and it is a mitochondria derived peptide involved in cellular energy and metabolic adaptation.

It has been studied for its potential effects on insulin sensitivity, glucose utilization, exercise capacity and metabolic flexibility.

The rationale between pairing MOTSI with five amino is that they approach metabolism through different but potentially complementary pathways.

So in simple terms, 5Amino 1 MQ may influence how fat cells store and expend energy, while Motts C can help support the mitochondria's ability to process available fuel.

I've done this stack.

I like this stack.

We'll talk more about my experience here shortly with this combination.

This combination is often discussed for metabolic health, insulin sensitivity, body recomposition, exercise performance, mitochondrial support, and overcoming weight loss plateaus.

Okay, NAD plus As we know, NAD is a coenzyme required for cellular energy production, mitochondrial function, DNA repair and sirtuin activity, and its relationship with 5Amino 1 and 2 is one of the more mechanistically logical pairings.

The strategy behind combining them is that NAD provides and supports cellular NAD.

5Amino 1 can reduce one pathway that diverts nicotinamide away from NAD recycling and together they can provide both NAD replenishment and NAD preservation.

So this increases NAD availability which can support mitochondrial energy production and metabolic signaling.

This pairing is common, commonly discussed for cellular energy, mitochondrial function, metabolic health, exercise recovery, healthy aging and body composition support.

I would say this is a good one if you're really needing a good energy boost, but you also don't want to overstimulate anything because sometimes that can have the opposite effect.

So you always want to try these things on their own before pairing them together.

I should have said that before I going down the stack thing.

But just know, just don't start all of these all at once cuz you can actually stack all the ones we're talking about together, but you definitely want to try them independently of each other first.

Okay.

This is a modified fragment of human growth hormone corresponding to Amino Acids176 through191 and it was developed to investigate the fat related effects of growth hormone with out reproducing its full growth promoting an IGF1 related activity.

The theory behind the combination is that AOD supports the mobilization of stored fat and 5amino1MQ can support the metabolic environment in which the fat is processed so it can help burn the fat.

So this combination can be attractive when the goal is body recomposition, whether than rather than just simply lowering scale weight.

Okay, glp last but not least.

So we all know what a GLP is, but this combination is good for people who are already losing weight with a GLP but want additional support for body composition, metabolic flexibility, fat loss plateaus.

I see it used a lot for that insulin sensitivity, energy during a calorie deficit and with help with muscle preservation.

So it's a good one for that which we all know is important on a GLP.

So who would be a good candidate for 5Amino 1?

If you want to improve your body composition or overcome fat loss plateaus which we just talked about, you want to support metabolic flexibility and insulin sensitivity, you want to reduce fat while preserving muscle.

You want to support energy and exercise performance, you want to improve cellular NAD metabolism and just add further metabolic support without appetite suppression, especially if you're on a glp.

And so I find that it works really well with all those things and with the people that I've worked with over the years, I, I really have seen it help people with fat loss stalls, not so much weight loss.

But if you're wanting to get your body composition in check, it's a really good one to pair with that to help get through those plateaus.

And I'll talk to you about my experience with that in just a minute.

But let's talk about what you're all here for and that is the dosing and it is all over the map.

And we have oral versus injectable.

So let's start with the oral version.

The oral capsules are the most commonly discussed route because 5Amino 1 did show some bioavailability in these rat models.

So the theory there is bioavailability in humans, although I've read mixed things about that.

I for one, I'll just throw this out there, am a believer in the the CEP Q route.

Although I have talked to people that swear by the oral route.

It just didn't work for me.

But I have heard that it's worked for people.

So if you have injection fatigue or you just don't want to inject, you want to try this, there are the oral versions out there.

It's just not one I'm a huge fan of.

But the conservative approach would be starting at 50 milligrams once daily, you want to take it in the morning and then you can cycle it for eight to 12 weeks.

A common range is between 50 milligrams to 100 milligrams.

Again, you want to take it in the morning.

And because it, it does provide you a lot of energy so you don't want to disrupt your sleep.

Although I've never experienced that.

And as if you've listened to me for any length of time, you know, I struggle with sleep.

And then the higher anecdotal range is 150 milligrams.

Sometimes this can be broken up into two doses.

And again, you want to do this for eight to 12 weeks.

I think this is where some of the confusion comes in.

I think that there's two things that are confusing.

You have these rat studies that were up to 60 milligrams per kilogram.

And so everyone thinks, oh, these super high doses injectable.

From the injectable standpoint, I'm like, look, if you want to take 50 milligrams, first of all, it's not really cost effective.

But if you want to do that daily, I would never tell you to do that.

I think that could potentially be dangerous just because we don't know enough about this.

But I've heard of people doing it.

What I think is interesting is that there are a lot of anecdotal evidence that you can get a really good benefit from five amino, much lower doses.

So I typically tell people to start out at 500mcgs to 1mg and see how you do, because you always want to stay on the lowest effective dose.

I've experimented up to 3mg daily.

Don't know that I saw a big difference between one to one and a half milligrams, which is normally the sweet spot where I stay.

And the 3 milligram dose.

Although in full disclosure, I've never done a dose that high for an extended period of time.

I've just tried it for a day or two, but I haven't noticed a big difference between those doses.

So my recommended dose is going to be start at least for the first couple of days at the 500mcgs, slowly move up.

If you feel like you need to, you don't have to.

I know I had someone report back to me recently in the school group, the Women's Peptide Collective, that I think she's a.

She's a cycle instructor and she was our spin instructor rather.

And she said that she was teaching her class and her energy was just off the hook high and she had just started 5amino 1 mq and that was at the 500 mcg, so the half a milligram mark.

So just like everything else, the dosing's going to be unique to the person, so you may have to play around with it a little bit.

The first time I started cycling 5 Omega 1MQ, maybe a year and a half to two years ago, I noticed that the lower dose immediately I just got really nice sustained energy.

And that was really the reason why I was taking it.

I like to cycle through NAD and then maybe do a MUTC and then maybe do a 5Amino 1 MQ.

Maybe before all that, do an SS31.

But I kind of cycle through all of these.

I don't normally take a bunch of them together, although I have done that if I'm particularly feeling rundown, something like that.

You might pair an SS31 and do an NAD or a MOT C and a 5Amino one MQ or NAD.

I have done that.

I feel like SS31 is just your basic foundational one.

And then you can stack a play around with these other ones after you've got that clean foundation established.

Although people do them together, I'm just telling you my preference.

And so I like to cycle through them individually.

So I have something working for energy and mitochondria and that sort of thing.

But I'm hitting it differently from different areas.

What I noticed was just this great sustained energy.

And then I got off of it and cycled a few other things and went back on it and I thought, oh, I'm gonna play around with the dose a little bit.

That's when I moved up to the one to one and a half, sometimes two milligrams of it.

And what was interesting to me is, yes, I had that great sustained energy.

I wouldn't say it was overwhelmingly more energy because I increased the dose, but I really leaned out in my midsection and I wasn't expecting that.

And it kind of hit me because I wasn't really doing anything else at the time.

I was on a low dose of GLP and I just my maintenance dose.

And I just noticed that my midsection, my waist was kind of getting more whittled in, my abs were getting a little more defined and I hadn't changed anything else.

And it struck me this is the five amino one.

So I'm not suggesting that you go out and do a higher dose and that's what's going to happen to you.

I will also say I work out very consistently.

I keep my nutrition very much in check and so it's not going to get you the six pack ripped abs if you're not, if those things aren't in play, if you're not working out and your nutrition isn't in check.

And some people just genetically that's not possible.

But what I did find was just a leaner waist, particularly in the midsection along with that beautiful sustained energy that I had throughout the day.

So it is definitely a favorite of mine along with MOT C and N A D and all of those and GLPs obviously, all the ones we kind of mentioned today.

And if you are in a stall or you're just wanting some nice energy or maybe you're wanting a little bit of some body recomp, particularly in that midsection, this might be one that you try.

If you haven't tried it, I think it's a good one to play around with.

And I'll go back to if you've tried it before, this is not a one size fits all.

We go off all this anecdotal information because we don't have the robust clinical trials like we do for the GLPs to know, hey, this is what works for the average population.

So we're out there in this world of I don't know, I'm going to try this, I'm going to try that.

Which is why the 50 milligram injection part of this came out of I don't know, I don't know where it came from.

But to me that just sounds absolutely crazy.

But don't give up on something just because you tried it once and it didn't work.

You may have to play around with it a little bit to figure out what works best for you because this is again one of my favorites and I've seen it work really well for a lot of people.

So we will just end on that note.

I hope you found this interesting.

If you have questions about it, always feel free to reach out to me.

Let's see what else.

Oh, you can find my email in the show notes.

Please email me there.

Do not email me at the Peptique.

My customer service team will promptly email you back and tell you that they cannot answer your questions.

But I can.

So feel free to do that.

If you are a woman, you hear me say this every time I release an episode and you want to know more about peptides or you know about peptides and you want to share information or you want to learn from a lot of other women.

Over 2,500 of them join the Women's Peptide Collective.

It's a free group that I started on the school platform.

You can find that link in the show notes as well.

Please put your full name and a little bit of a bio, because if you don't do that, we get a lot of spam people that try to get in there.

I will reject your membership.

So just know that and let's see what else.

I think that's it.

Sick and bears.

I hope everyone's enjoying the new football season.

And until next time, everyone have a blessed day.